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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Immunology and Serology | 5-10% | - Infectious disease serology - Autoimmune and immunodeficiency disorders - Immunologic testing methodologies - Immune system principles and components |
| Urinalysis and Other Body Fluids | 5-10% | - Semen and amniotic fluid testing - Urine formation and composition - Cerebrospinal, serous, and synovial fluids - Physical, chemical, and microscopic analysis |
| Blood Banking (Immunohematology) | 15-20% | - Transfusion reactions and adverse events - Compatibility testing - Blood components and storage - Blood group systems and antigens - Antibody detection and identification |
| Microbiology | 15-20% | - Bacteriology and antimicrobial susceptibility - Specimen collection and culture techniques - Identification of pathogenic microorganisms - Mycology, parasitology, and virology |
| Hematology | 20-25% | - Routine and automated hematology procedures - White blood cell morphology and diseases - Hemostasis and coagulation testing - Red blood cell physiology and disorders |
| Laboratory Operations | 5-10% | - Quality control and quality assurance - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance - Pre-analytical, analytical, and post-analytical processes |
| Clinical Chemistry | 20-25% | - Carbohydrates, lipids, proteins, and enzymes - Toxicology and liver/kidney function tests - Electrolytes, acid-base balance, and blood gases - Endocrinology and therapeutic drug monitoring |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Certain recipients have increased risk for developing TA-GVHD. They are:
Neonates less than 4 months of age
Fetuses
Recipients with a congenital or acquired immunodeficiency, such as bone marrow or stem cell recipients, and patients receiving chemotherapy recipients of donor units from a blood relative Which of the following patients are at risk for transfusion-associated graft versus host disease (TA-GVHD) and require irradiated cellular blood products? (Choose all that apply)
A) Patient receiving chemotherapy who are immunocompromised.
B) Neonates less than 4 months of age
C) Patients with a history of allergic reactions.
D) Recipients of donor units known to be from a blood relative.
E) Patients with chronic anemias.
2. The ratio of blood to anticoagulant in a light blue stopper tube is:
A) 10:1
B) 5:1
C) 9:1
D) 2:1
3. 1. B
2. D
3. A
4. C
Red to Brown Urine: porphobilinogen, hematuria, myoglobinuria, etc.
Green: Food colorings; Increased carotene in the diet;
Pseudomonas aeruginosa infection
Yellow: bilirubin, bile pigments
White: phosphates, other crytals
Match urine color with substance that might have been responsible:
1. Phosphates
2. Bilirubin
3. Pseudomonas
4. Porphobilinogen
A) Blue to green
B) Red to brown
C) White
D) Yellow
4. G6PD deficiency causes an increased suseptibility to the oxidation of hemoglobin, which in turn, causes the precipitation of hemoglobin inside of the red blood cell.
Which one of the following conditions is associated with glucose-6-phosphate dehydrogenase (G6PD) deficiency?
A) Faulty heme synthesis
B) Hemoglobins with low oxygen affinities
C) Precipitation of hemoglobin
D) Microcytic red cells
5. FAB M5 is acute monoblastic leukemia
FAB M1 is acute myeloblastic leukemia without maturation
FAB M3 is acute promyelocytic leukemia
FAB M4 is acute myelomonocytic leukemia
Which FAB designation is called the "true" monocytic leukemia and is characterized by monoblasts, promonocytes, and monocytes?
A) FAB M5
B) FAB M4
C) FAB M3
D) FAB Ml
Solutions:
| Question # 1 Answer: A,B,D | Question # 2 Answer: C | Question # 3 Answer: A,B,C,D | Question # 4 Answer: C | Question # 5 Answer: A |






