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American Society of Microbiology ABMM Sample Questions:

1. A microbiology laboratory is performing Gram staining on a bacterial smear. If the safranin counterstain is omitted, Gram-negative bacteria will appear:

A) Brown
B) Purple
C) Colorless
D) Blue

2. A clinical trial is evaluating the efficacy of a new antifungal agent against invasive aspergillosis. Serum galactomannan levels are being used as a biomarker to monitor the infection. A decrease in galactomannan levels during treatment would indicate:

A) Successful clearance or reduction of the Aspergillus burden.
B) Development of resistance to the antifungal agent.
C) A false-positive result in the galactomannan assay.
D) Concurrent infection with a non-Aspergillus mold.

3. A researcher is investigating the mechanism of action of a novel antibacterial agent that inhibits bacterial protein synthesis. The agent binds to the 50S ribosomal subunit and prevents the translocation of peptidyl- tRNA. This mechanism of action is similar to which of the following antibiotics?

A) Ciprofloxacin
B) Penicillin
C) Vancomycin
D) Azithromycin

4. The erm gene in Staphylococcus aureus confers resistance to which class(es) of antibiotics via modification of the ribosomal target site?

A) Beta-lactams only
B) Fluoroquinolones
C) Macrolides, Lincosamides, and Streptogramin B (MLS<sub>B</sub>)
D) Aminoglycosides

5. A microbiology laboratory receives a CSF sample from a patient with suspected fungal meningitis. India ink staining reveals encapsulated yeast cells. Culture on Sabouraud dextrose agar grows creamy white colonies.
The MOST likely organism is:

A) Cryptococcus neoformans
B) Mucor species
C) Aspergillus flavus
D) Candida albicans

Solutions: